RAPID CLEAR CAP 3000
High-speed clarification of cell culture
還在用傳統濾杯過濾培養液嗎?
不先把細胞分裝離心,濾杯過濾很快就塞住了...
過濾救星! THOMSON RAPID CLEAR CAP 3000 學士帽造型濾杯!
0.2 μm filter直接接在搖瓶上,免分裝離心,免倒出,快速又安全!
省時省錢! 省下用離心機的時間與開銷
省下13倍以上的時間!
一個學士帽抵 4個 !! 500 cc濾杯
搭配Optimum GrowthTM Flask搖瓶,瓶頸加寬,高通氣低剪切力,產量更好!
參考的使用體積與所需時間 註: 不同細胞總類與濃度,所需時間會略有不同
| Cell Line Viability | 99 %-70 % | 69 %-50 % | 49 %-40 % | 39 %-0 % Spin for 7 min @ 4000 g* |
||||
| Cell Type | Volume (L) | Time (min) | Volume (L) | Time (min) | Volume (L) | Time (min) | Volume (L) | Time (min) |
| CHO Stable without Feed | 3.0 | 18 | 2.5 | 18 | 2.0 | 20 | 3.5** | 35** |
| CHO Stable, 1 to 2 Feeds | 2.0 | 18 | 2.0 | 18 | 1.5 | 35 | ||
| CHO Stable, 2+ Feeds | Spin for 15 min @ 3000 g; 3 L volume ** | |||||||
| HEK293 (FreeStyleTM & Expi293) | 3.0 | 18 | 3.0 | 23 | 3.0 | 25 | 3.5** | 35** |
| CHO Transient | 3.0 | 18 | 2.5 | 18 | 1.5 | 35 | ||
| ExpiCHO | 3.0 | 18 | 2.5 | 18 | 1.0 | 18 | ||
* For low viability cultures, (< 39%), centrifuge for 7 minutes prior to clarifying with the Rapid Clear Cap 3000.
** Cell cultures that received 2+ feeds will require spinning to minimize potential clogging
實作影片
想要增進實驗效率嗎? 趕快來電詢問!!
若有更多過濾問題,也歡迎於部落格留言與我們討論,或參考
THOMSON代理-欣梗科技股份有限公司
Tel: 02-28321066
E-mail: info@lefoscience.com
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